WebBD Pharmingen™ MitoStatus TMRE labeling of cells for fluorescent imaging 1. Stain cells in fresh, pre-warmed media with 20-200 nM BD Pharmingen™ MitoStatus TMRE. 2. Incubate samples for 15-30 minutes at 37°C protected from light. 3. Remove the staining solution and replace with pre-warmed DPBS. 4. Analyze cells by fluorescence imaging. WebTMRM signal can be detected with fluorescence microscopy, flow cytometry, cell sorting, high throughput screening, and high content analysis. TMRM has the following properties: …

MitoProbe™ TMRM Assay Kit for Flow Cytometry

WebTMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells Contains TMRE, FCCP control and the assay buffer. 100 tests or 500 test format for microwells avaialble. Stain only live cells and does not work on fixed cells. Suitable for fluorescence microscopy, fluorometry or flow cytometry. WebTMRE emits a red fluorescence that can be detected by flow cytometry or fluorescence microscopy and the level of TMRE fluorescence in stained cells can be used to determine … how fer is homosexaulity in the country

TMRE Mitochondrial Membrane Potential Assay │ G-Biosciences

WebTMRM and TMRE signals can be detected in live cells or isolate mitochondria with traditional and high content fluorescence microscopy, microplates, or by flow cytometry (Figure 2). … WebProtocols using MitoPT ® TMRE or TMRM at concentrations greater than 50 nM should include a single wash step to minimize background ! uores-cence. When viewing MitoPT … Websolution. Each vial includes enough TMRE for five 96-well plates (0.1 µl/well) or 50 flow cytometry assays (10 µl/assay). Aliquot if desired and store at -20°C. Dilute TMRE to 1:500 with full cell culture medium to make 10X TMRE Labeling Solution. 2 µM is used in this protocol; 0.1 to 10 µM is recommended based on different cell lines. 3. how feral am i